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About This GigaPanToggle
- Taken by
- Aresty Posters 2009
- Explore score
- 0.06 Gigapixels
- Date added
- June 03, 2009
- Date taken
- June 02, 2009
Title: Roles of Nkx6.1 in Regulating Notch1 Expression During Early Development
Deciphering the function of Nkx-6.1 in regulating Notch1 expression during nerve development may prove useful in the development of therapies for the treatment of nervous system-related health conditions. NKX-6.1’s gene product, the protein Nkx-6.1, is a 367 amino acid transcription factor that contains a 60 amino acid DNA-binding domain, which is thought to interact with Notch1 gene during early development. In this study, we establish Nkx6-1 as a key regulator for Notch1 expression through a novel enhancer by verifying the binding of Nkx-6.1 to the Notch1-Conserved Region 2 (Notch1-CR2) using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay. Furthermore, to show the role of Nkx-6.1 in cell fate determination of neural stem cells through its regulation of Notch1, Nkx-6.1 was both over and under-expressed in chick embryo to gain insight on the protein’s specific function both spatially and temporally. These alternations in the expression pattern seem to clearly convey the importance of Nkx-6.1 in regulating the Notch1 expression and thereby determining stem cell differentiation. Our findings will help decode developmental mechanisms that will provide a basis for the development of potential stem cell treatments or therapeutic transplants for diseases ranging from neural degeneration disease to spinal cord injury.
Tapan Patel, Evangeline Tzatzalos, Jennifer Forino, Sung Tae Doh, and Li Cai
Rutgers, Biomedical Engineering